My recent publication:
Authors:
Sanjiv
Kumar, Bhanwar Lal Puniya, Shahila Parween, Pradip Nahar, Srinivasan
Ramachandran
Abstract
Pathogenic
bacteria interacting with eukaryotic host express adhesins on their surface.
These adhesins aid in bacterial attachment to the host cell receptors during
colonization. A few adhesins such as Heparin binding hemagglutinin adhesin
(HBHA), Apa, Malate Synthase of M. tuberculosis have been identified using
specific experimental interaction models based on the biological knowledge of
the pathogen. In the present work, we carried out computational screening for
adhesins of M. tuberculosis. We used an integrated computational approach using
SPAAN for predicting adhesins, PSORTb, SubLoc and LocTree for extracellular
localization, and BLAST for verifying non-similarity to human proteins. These
steps are among the first of reverse vaccinology. Multiple claims and attacks
from different algorithms were processed through argumentative approach.
Additional filtration criteria included selection for proteins with low
molecular weights and absence of literature reports. We examined binding
potential of the selected proteins using an image based ELISA. The protein
Rv2599 (membrane protein) binds to human fibronectin, laminin and collagen.
Rv3717 (N-acetylmuramoyl-L-alanine amidase) and Rv0309 (L,D-transpeptidase)
bind to fibronectin and laminin. We report Rv2599 (membrane protein), Rv0309
and Rv3717 as novel adhesins of M. tuberculosis H37Rv. Our results expand the
number of known adhesins of M. tuberculosis and suggest their regulated
expression in different stages.
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